Question 1

How is the scale bar width calculated?

Accepted Answer

The displayed width in pixels is the scale bar length divided by the physical size per pixel.

Question 2

Can calibration be read automatically?

Accepted Answer

OME-TIFF and ImageJ TIFF calibration can be detected. Other images require a physical size per pixel.

Question 3

How is the bar color selected?

Accepted Answer

The tool checks the image area behind the bar and initially chooses black or white for contrast. You can change both colors manually.