Tools

Culture vessel A
cm²
Seeding density D
Target cells per wellN = D × A--
Cell suspension per wellVₛ = N / Cₛ-- mL
Medium per wellVₘ = V − Vₛ-- mL
Total cell suspensionVₛ,total = Vₛ × n-- mL
Total mediumVₘ,total = Vₘ × n-- mL